version 5.0, august 2012. objectives. 6 0 obj in practice: collecting specimens and samples. They have retained the strong carbol fuchsin dye that has penetrated the cells following heating of the slide to steam heat, even though the film has been decolourised with a mixture of acid and alcohol. 1 0 obj Specimen Collection - . Low-Flow Nebulizers.
WebSPECIMEN COLLECTION Ask him/her to breath in third time and then forcefully blow the air out.
Even if you have a relatively good knowledge of the local flora, you may not be able to accurately identify all plants in the field. Specimen Collection - . Rough colonies do not generally cause disease. Campylobacters have only been recognised as human pathogens since the late 1970's, although they have been long considered to be animal pathogens. Note: On cysteine heart agar, F tularensis colonies are characteristically opalescent and do not discolor the medium (Dennis et al., 2001). materials for specimen collection. There are two major groups of Gram-positive cocci that are of medical importance: the staphylococci and the streptococci . LM X500, Conventional Biochemical tubes for Shigella spp, Conventional Biochemical tubes for Salmonella spp.
| PowerPoint PPT presentation | free to view Description: Gram Stain Smears of the Agents of Anthrax (Bacillus anthracis), Plague (Yersinia pestis), and Tularemia (Francisella tularensis), Demonstrating Comparative Morphology, Size, and Staining Characteristics (Dennis et al., 2001). | PowerPoint PPT presentation | free to view The used swab will be then put into the beaker with the disinfectant on the table. may be performed by a physician at the patients, Laboratory Diagnostics and Specimen Collection - .
Gram-negative bacilli Enterobacteriaceae This image shows the typical appearance of Escherichia coli when stained using the Gram staining method The Enterobacteriaceae are a large family of medically important Gram-negative bacilli.
Tularemia, also known as rabbit fever or deer-fly fever, is a zoonotic disease caused by the gram-negative intracellular pathogen Francisella tularensis (Farlow et al., 2005. Members of the recently recognised genus Legionella, some of which cause atypical pneumonias like Legionnaires' disease, require higher levels of iron and cysteine than are usually present in bacteriological media, and they grow best in media that incorporate activated charcoal to adsorb their toxic metabolic products. Specimen Collection and Transport The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). %PDF-1.5
3. PG,MEDICAL MICROBIOLOGY 3yr. heather nelson, rn. endobj
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Some streptococci have no effect on blood.
specimen collection, preservation and transportation ppt Impact of Incorrect Volume on PT and APTT The APTT is the most sensitive to variations in final citrate concentration. gram reaction, Since septicemia and complications thereof has, The results must be communicated to the physician, Lab results on cerebrospinal fluid are also, Several bacterial species (and other pathogens), Diarrhea can be limited to cramping loose, Enterotoxins attach to the intestinal mucosal, Other exotoxins (see conditions on next slide), Colitis involves diarrhea with additional, Enterocolitis is similar but also involves the, Dysentery includes the above symptoms along with, These and other GI conditions are much less, Culture and direct wet preparations for fecal, Through further study and improved methods it was, Enteric pathogens that emerged include Salmonella, Collection of fecal samples is relatively, Feces (stool) is the specimen of choice for, Stool should be collected in a wide-mouth, If the patient cannot produce a stool specimen, Swab samples are generally processed more rapidly, It is always best to transport all samples, Stool that cannot be processed within one hour, If intestinal parasites are suspected, samples, The majority of GI infections are caused by, Therefore a Gram stain for bacterial cellular, Fecal Gram stains for leukocytes (PMNs) are, A Methylene blue wet prep is an easy alternative, The presence of Gram () cocci in clusters, A preponderance of budding cells (with or without, Campylobacter is a tiny curved or loosely, Fecal specimens are heavily contaminated with, Fecal microbes, in general, are resistant to, This allows application of various selective and. Gram stain One of the most important techniques in bacteriology is the Gram stain, first described by Hans-Christian Gram in 1884. WebSPECIMEN COLLECTION Ask him/her to breath in third time and then forcefully blow the air out. multiple methods (routine diagnostic, serology, bacteremia is sometimes transient growth may, unless multiple samples are collected, it is, Sufficient quantity of specimen is required, Swabs, due to the small volume collected and the, The quicker the specimen is processed the better, Pathogens may die in transit thus leading to, If transport is delayed, a transport medium, a reducing agent such as thioglycolate can be, CO2 can be included to support viability of, Charcoal, gelatin, or corn starch can be included, Buffered glycerol is sometimes used to transport, Boric acid will preserve urine specimens for, Refrigerated urine for culture can be kept up to, Some microbes do not survive well even in, Neisseria gonorrhoeae lose viability genital, If swabs must be used the following guidelines, Cotton may contain toxins to fastidious pathogens, Calcium alginate inhibits Chlamydia and other, Dacron (polyester) is the best all-around, The upper respiratory tract (URT) includes the, The oral cavity, oropharynx nasopharynx, are, The eustachian tube, middle ear, and the nasal, The principle of collecting a throat specimen is, A tongue blade should be used to press down on, The throat should be visualized using good, Areas in the posterior pharynx that appear, The tonsilar fossae and the extreme posterior. <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Some microbiologists think that this gives them the microscopic appearance of Chinese letters. Whatever your area of interest, here youll be able to find and view presentations youll love and possibly download. <>
Therefore, it is necessary to purchase FDA approved products that comply with quality standards. Specimen Collection and Transport. Streptococcus pyogenes and shows Gram-positive cocci again, this time in chains. materials for specimen collection.
Our new CrystalGraphics Chart and Diagram Slides for PowerPoint is a collection of over 1000 impressively designed data-driven chart and editable diagram s guaranteed to impress any audience. Microbiology 101 and Specimen Collection - . They appear somewhat irregular in shape, and tend to cluster in Gram films. jumphol mitchai md department of pathology kku. - Laboratory Diagnostics, Specimen Collection, and Biosafety Issues * Module 5: Laboratory Diagnosis, Specimen Collection, and Biosafety Issues * When investigating Electronic Toll Collection Market Development Strategy and Future Prospects 2023, - Market Research Future published a research report on Electronic Toll Collection Market Research Report - Global Forecast to 2023 Market Analysis, Scope, Stake, Progress, Trends and Forecast to 2023. Members of this genus are increasingly associated with hospital-acquired infection. Each specimen type requires specific packing and handling procedures. Boasting an impressive range of designs, they will support your presentations with inspiring background photos or videos that support your themes, set the right mood, enhance your credibility and inspire your audiences.
- Recommended Procedures for Sample Collection, Preservation and Shipping Dennis A. Senne dennis.a.senne@aphis.usda.gov (515) 663-7551 USDA, APHIS, VS, Preliminary Planning for an International Mars Sample Return Mission, - Preliminary Planning for an International Mars Sample Return Mission iMARS Working Group, Lecture 2: Collection and Preservation of Samples, - The Islamic University of Gaza- Environmental Engineering Department Environmental Measurements (EENV 4244) Lecture 2: Collection and Preservation of Samples, - AIR FREIGHT TRANSPORT MANAGEMENT Fresh produce Arab Academy for Science & Technology & Maritime Transport Institute Of International Transport & Logistics. Sample collection for bacterial isolation, characterization and antibiotic se TB_Specimen_Collection_thru_Processing_TrainerNotes.pdf, Collection and transport of clinical samples in laboratorries, Specimen collection 130319145806-phpapp02, Lab sample collection techniques pathology, Specimen collection for clinical microbiology laboratory, Collection of biological specimens for microbiology tests, Specimen collection and transport Dr.Ashna Ajimsha, Blue Gradient Company Business Profile Presentation.pdf, integratedskills-130107151920-phpapp01 (1).pdf, finalfdiinretailppt-101011161658-phpapp01 (1).pptx, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. Mixed bacterial colonies on MacConkey agar, Escherichia coli (red) and Salmonella typhimurium (white). PowerPoint PPT presentation, Virology Specimen Collection MarketReport 2022- Market Size, Trends, And Global Forecast 2022-2031. WebVirology Specimen Collection Market Report 2022- Market Size, Trends, And Global Forecast 2022-2031 - TBRC global virology specimen collection market report includes blood collection kits, specimen collection tubes, viral transport media, swabs. When results regularly fall outside this range, they should be regarded as evidence that a technical error has been introduced into the test, or that the reagents are at fault. 4. know the basic components of specimen collection, Common Lab Tests Specimen Collection - The role of the nurse in specimen collection. During vegetation sampling, collect repre- X and V dependence may also be demonstrated by the phenomenon of 'satellitism'. To visualise any other material on the slide, methylene blue is applied as a counterstain. Gram-positive bacteria have many layers of peptidoglycan in their cell wall; Gram-negative bacteria have only one or two peptidoglycan layers but, additionally, they have an outer membrane. jitendra kumar pandey,mgm medical college,mumbai. These are the non-haemolytic streptococci (see below). - Specimen Collection Laboratory specimen: collection, safe transport and biosafety * Slide * Sputum This indicates how to collect sputum. And theyre ready for you to use in your PowerPoint presentations the moment you need them. The bacterium Helicobacter pylori, found associated with stomach ulcers, is a good example of a bacterium that has undergone a radical change in taxonomy.
RT-PCR TESTING CAN TAKE 48 -72 HOURS FROM THE TIME THE SPECIMEN IN PICKED UP BY THE LABORATORY.
(Haemophilus influenzae) and (Staphylococcus) satellite test (Haemophilus influenzae) and (Staphylococcus aureus) on blood agar, culture (Haemophilus influenzae) and (Staphylococcus) satellite test, Quality control procedures for commonly used tests, Quality control procedures It is also essential to undertake quality control procedures at regular intervals. Ask him/her to breath in again and then cough. refer to nursing care guidelines 52-1. Webspecimen collection, preservation and transportation ppt dr. mervat, Establishing diagnostic microbiology laboratory - . The colonies are surrounded by haloes that are completely clear. As their names imply, these bacteria can be found in the gut, and can grow in the presence of bile salts. The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). WebSPECIMEN COLLECTION Ask him/her to breath in third time and then forcefully blow the air out. F. tularensis grows best in cysteine-enriched broth and thioglycollate broth. WebINTORDUCTION: Specimen collection and transportation are critical considerations , because any results the laboratory generates is limited by the quality of the specimen and its condition on arrival in the laboratory. Webthe desired tests at the place of collection or laboratory located far away, request the Diagnostic laboratories to advice on transportation of specimens, and consider how When to Request Transport Medium to preserve and transport in ideal medium before it is processed Popularly used transport medium are-Amie 's transport Medium: Web5-3: Collection and preservation The collection of appropriate and optimum samples is the responsibility of the laboratory, even though the actual collection process is often carr These bacteria also grow best on media that contain activated charcoal. laboratory tests are important in helping to diagnose patient problems. The family Enterobacteriaceae include Yersinia pestis, the cause of plague, Salmonella typhi, the cause of typhoid, Shigella dysenteriae, the cause of bacilliary dysentery, and Salmonella enteritidis implicated in many cases of food poisoning.
Francisella tularensis Growth at 72 Hours After Inoculation These Francisella tularensis colonies show characteristic opalescence on cysteine heart agar with sheep blood (cultured at 37 C for 72 hours).
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Each reagent and each step in the test should then be investigated until the cause of the error has been found and eliminated, 2023 SlideServe | Powered By DigitalOfficePro, - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -. in practice: collecting specimens and samples. More info:- https://www.imarcgroup.com/automated-fare-collection-market, Architecture of the Transport Telematics Systems, - Architecture of the Transport Telematics Systems Doc. endobj
specimen collection, preservation and transportation ppt. Ask him/her to breath in again and then cough. WINTER Refrigerate immediately at 4C, and then transport in a cold box. viral transport media in collection tubes, Specimen collection - . Ask the patient to close the Staphule is Greek for grapes. An inoculum of Staphylococcus aureus, which can provide NAD, is placed on the plate and the culture is incubated. Specimens should be collected from the areas where organisms are present. Wipe off the obtained swab on the surface of the blood and make the inoculum . If so, just upload it to PowerShow.com. Webcollection will need to be explained in detail in the instructions for collection. Incubate the inoculated plate at 37oC for 24 hours and put plates "upside down" to prevent condensation of water on the surface of the agar. Any important safety precautions should be explained. Spores of Gram-positive rods are highly resistant structures that may add considerably to their pathogenic capacity. Specimen Integrity is dependent on accurate pre-analytical processes to include patient preparation, specimen collection, handling, and transportation. viral transport media in collection tubes, Specimen collection - . specimen collection. sandy villar nca, ascp microbiology supervisor anmc. Clostridium tetani Bacteria are gram-postive rods or bacilli with terminal spores that cause tetanus in humans, spore.